The Copenhagen Soccer Test: Physiological response and fatigue development

Mads Bendiksen, Rasmus Bischoff, Morten B. Randers, Magni Mohr, Ian Rollo, Charlotte Suetta, Jens Bangsbo, Peter Krustrup

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)


Introduction: The aims of the study were 1) to evaluate whether a multifaceted simulated soccer game protocol, entitled the Copenhagen Soccer Test (CST), elicited a similar physiological loading as a competitive game (CG) and 2) to determine muscle metabolites, blood variables, and sprint performance in various phases of CST. Methods: Twelve Danish Second- and Third-Division soccer players participated in the study. On separate days, HR measurements, frequent blood sampling, and physical/technical tests were performed during 60- and 90-min versions of the CST during which repeated musculus vastus lateralis biopsies were collected. A CG was also played, where HR was recorded and pre- and post-game muscle biopsies and blood samples were collected. Results: No differences were observed between CST and CG in average HR (85% T 1% and 86% T 1% HRmax, P 9 0.05) or recovery plasma creatine kinase (24 h: 312 T 57 and 324 T 76 UILj1 , P 9 0.05). Muscle glycogen decreased (P G 0.05) from 459 T 15 to 232 T 30 mmolIkgj1 dry weight (d.w.) during CST, which was not different from CG (P 9 0.05). The rate of glycogen utilization was 4 T 1 mmolIkgj1 d.w.Iminj1 during the warm-up and the first 15 min of CST and 1 T 1 mmolIkgj1 d.w.Iminj1 (P G 0.05) from 60 to 90 min of CST. After 15 min of CST, muscle lactate was elevated (P G 0.05) approximately fivefold (24 T 3 mmolIkgj1 d.w.), and creatine phosphate was lowered (P G 0.05) by È60% (28 T 4 mmolIkgj1 d.w.). Sprint velocity (2 20 m) decreased (P G 0.05) by 7% during CST (5.2 T 0.6 to 4.9 T 0.7 mIs j1 ). Conclusions: The physiological response to the CST was reproducible and comparable to that of high-level CG. The CST allowed for rapid muscle sampling and revealed high creatine phosphate degradation throughout the test and a lowered glycogen utilization toward the end of the simulated game.
Original languageEnglish
Pages (from-to)1595-1603
Number of pages9
JournalMedicine and Science in Sports and Exercise
Issue number8
Publication statusPublished - Aug 2012


  • Muscle glycogen utilization
  • muscle crp
  • lactate
  • plasma ck
  • sprint performance
  • association football


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