MassSorter: peptide mass fingerprinting data analysis

Ingvar Eidhammer, Harald Barsnes, Svein-Ole Mikalsen

Research output: Chapter in Book/Report/Conference proceedingChapter


MassSorter is a software tool that sorts, systemizes, and analyzes data from peptide mass fingerprinting (PMF) experiments on proteins with known amino acid sequences. Several experiments can be simultaneously analyzed for sequence coverage and posttranslational modifications occurring during sample handling, induced chemical modifications, and unexpected cleavages. Experimental m/z values are compared with m/z values from an in silico digestion, taking modifications into account. Filters can be defined by users for marking autolytic protease peaks and other contaminating peaks. MassSorter functions as a database of all the detected peptides. It includes tools for visualization of the results, such
as sequence coverage, accuracy plots, statistics, and 3D models.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationFunctional Proteomics - Methods and Protocols
EditorsJulie D. Thompson, Christine Schaeffer-Reiss, Marius Ueffing
PublisherHumana Press / Springer Science Media
Number of pages16
ISBN (Print)978-1-58829-971-0
Publication statusPublished - 2008
Externally publishedYes


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