Effects of high-intensity intermittent training on potassium kinetics and performance in human skeletal muscle

Jens Jung Nielsen, Magni Mohr, Christina Klarskov, Michael Kristensen, Peter Krustrup, Carsten Juel, Jens Bangsbo

Research output: Contribution to journalArticlepeer-review

123 Citations (Scopus)

Abstract

A rise in extracellular potassium concentration in human skeletal muscle may play an important role in development of fatigue during intense exercise. The aim of the present study was to examine the effect of intense intermittent training on muscle interstitial potassium kinetics and its relationship to the density of Na+,K+-ATPase subunits and KATP channels, as well as exercise performance, in human skeletal muscle. Six male subjects performed intense one-legged knee-extensor training for 7 weeks. On separate days the trained leg (TL) and the control leg (CL) performed a 30 min exercise period of 30 W and an incremental test to exhaustion. At frequent intervals during the exercise periods interstitial potassium ([K+]I) was determined by microdialysis, femoral arterial and venous blood samples were drawn and thigh blood flow was measured. Time to fatigue for TL was 28% longer (P < 0.05) than for CL (10.6 ± 0.7 (mean ±s.e.m.) versus 8.2 ± 0.7 min). The amounts of Na+,K+-ATPase α1 and α2 subunits were, respectively, 29.0 ± 8.4 and 15.1 ± 2.7% higher (P < 0.05) in TL than in CL, while the amounts of β1 subunits and ATP-dependent K+ (KATP) channels were the same. In CL [K+]I increased more rapidly and was higher (P < 0.05) throughout the 30 W exercise bout, as well at 60 and 70 W, compared to TL, whereas [K+]I was similar at the point of fatigue (9.9 ± 0.7 and 9.1 ± 0.5 mmol l−1, respectively). During the 30 W exercise bouts and at 70 W during the incremental exercise femoral venous potassium concentration ([K+]v) was higher (P < 0.05) in CL than in TL, but identical at exhaustion (6.2 ± 0.2 mmol l−1). Release of potassium to the blood was not different in the two legs. The present data demonstrated that intense intermittent training reduce accumulation of potassium in human skeletal muscle interstitium during exercise, probably through a larger re-uptake of potassium due to greater activity of the muscle Na+,K+-ATPase pumps. The lower accumulation of potassium in muscle interstitium in the trained leg was associated with delayed fatigue during intense exercise, supporting the hypothesis that interstitial potassium accumulation is involved in the development of fatigue.
Original languageEnglish
Pages (from-to)857-870
Number of pages14
JournalThe Journal of physiology
Volume554
Issue number3
DOIs
Publication statusPublished - 2004

Keywords

  • high-intensity intermittent training
  • skeletal muscle
  • potassium

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